Purification of tritium-labeled cholera toxin.

نویسندگان

  • J G Banwell
  • D W Hanke
  • D Diedrich
چکیده

Cholera toxin was labeled with tritium by the Wilzbach technique, and highly purified radiolabeled toxin was obtained by Sephadex column chromatography and disc gel electrophoresis. 3H-labeled cholera toxin retained its biological activity and chemical stability and had a specific activity of 405.9 muCi/mumol. The methods utilized in extraction and purification of 3H-labeled toxin may be advantageous for preparation of other biologically active radiolabeled proteins.

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عنوان ژورنال:
  • Infection and immunity

دوره 21 2  شماره 

صفحات  -

تاریخ انتشار 1978